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Peptide and Protein Chemistry and Engineering Group

Logo zespołu Chemii i Inżynierii Peptydów i Białek

The main scientific interests of the Peptide and Protein Chemistry and Engineering Team focus on the synthesis, research, and application of protein fragments and synthetic peptides. Utilizing peptide synthesis methods and their derivatives, the team aims to create peptide collections that resemble the properties of native proteins. The selection of active protein fragments enables the use of peptides in medicine, biomedical engineering, and biology. The group is led by Prof. Beata Kolesińska.

Key research areas include:

  • Design and synthesis of biologically active peptides and their application in medicine.
  • Design, synthesis, and utilization of peptide conjugates (peptide-drug, peptide-marker).
  • Design and production of peptide/protein materials with defined activity and utility.
  • Design and creation of hybrid materials containing peptides and/or proteins with intended biological activity.
  • Study of peptide 3D structures and the relationship between their conformation and susceptibility to forming stable conformers that favor the initiation of the aggregation process.
  • Proteomics and metabolomics studies using mass spectrometry.

 

Treść (rozbudowana)
Selected Papers

    1. Czerczak-Kwiatkowska K., Kaminska M., Fraczyk J., Majsterek I., Kolesinska B., “Searching for EGF Fragments Recreating the Outer Sphere of the Growth Factor Involved in Receptor Interactions” Int. J. Mol. Sci. (2024), 25, 1470. https://doi.org/10.3390/ijms25031470

    2. Wasko J., Wolszczak M., Zajaczkowska Z., Dudek M., Kolesinska B., „Human serum albumin as a potential drug delivery system for N-methylated hot spot insulin analogs inhibiting hormone aggregation” Bioorganic Chemistry 143 (2024) 107104

    3. Baran K., Wasko J., Kryczka J., Boncela J., Jabłonski S., Kolesinska B., Brzezianska-Lasota E., Kordiak J., “The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients” Int. J. Mol. Sci. (2023), 24, 13669.

    4. Sikorski D., Rosiak P., Janczewski Ł., Potrzebowski M.J., Kregiel D., Kaźmierski S., Neubauer D., Kolesińska B., Frączyk J., Adamczyk A., Draczyński Z., “Synthesis and Characterization of Antibacterial Chitosan Films with Ciprofloxacin in Acidic Conditions” Int. J. Mol. Sci. (2023), 24, 15163.

    5. Gałecki K., Kowalska-Baron, A., Nowak, K.E., Gajda A., Kolesinska B., “Steady-State and Time-Resolved Fluorescence Study of Selected Tryptophan-Containing Peptides in an AOT Reverse Micelle Environment” Int. J. Mol. Sci. (2023), 24, 15438.

    6. . Becht A., Frączyk J., Wasko J., Menaszek E., Kajdanek J., Miłowska K., Kolesinska B., „Selection of collagen IV fragm ents form ing the outer sphere of the native protein: Assessment of biological activity for regenerative medicine” J. Pept. Sci. (2023), e3537

    7. Kolasa M., Czerczak K., Fraczyk J., Szymanski L., Lewicki S., Bednarowicz A., Tarzynska N., Sikorski D., Szparaga G., Draczynski Z., Cierniak S., Brzoskowska U., Galita G., Majsterek I., Bociaga D., Krol P., Kolesinska B. Evaluation of Polysaccharide–Peptide Conjugates Containing the RGD Motif for Potential Use in Muscle Tissue Regeneration Materials (2022), 15, 6432.

    8. Kryczka J., Janczewski Ł, Gajda A., Frączyk A., Boncela J., Kolesińska B., Brzeziańska-Lasota E., „Isothiocyanates (ITCs) 1-(Isothiocyanatomethyl)-4-phenylbenzene and 1-Isothiocyanato-3,5-bis(trifluoromethyl)benzene—Aldehyde Dehydrogenase (ALDH) Inhibitors, Decreases Cisplatin Tolerance and Migratory Ability of NSCLC” Int. J. Mol. Sci. (2022), 23, 8644.

    9. Tryba A.M., Krok-Borkowicz M., Kula M., Piergies N., Marzec M., Wegener E., Fraczyk J., Jordan R., Kolesinska B., Scharnweber D., Paluszkiewicz C., Pamuła E., “Surface Functionalization of Poly(L-lactide-co-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering” J. Funct. Biomater., (2022), 13, 4.

    10. Kolasa M., Galita G., Majsterek I., Kucharska E., Czerczak K., Wasko J., Becht A., Fraczyk J., Gajda A., Pietrzak L., Szymanski L., Krakowiak A., Draczynski Z., Kolesinska B., “Screening of Self-Assembling of Collagen IV Fragments into Stable Structures Potentially Useful in Regenerative Medicine” Int. J. Mol. Sci., (2022), 22, 13584.

    11. . Rosiak P., Latanska I., Paul P., Sujka W., Kolesinska B., “Modification of Alginates to Modulate their Physic-Chemical Properties and Obtain Biomaterials with Different Functional Properties” Molecules, (2021), 26, 7264.

    12. Matusiak M., Rurarz B.P., Kadłubowski S., Wolszczak M., Karczmarczyk U., Maurin M., Kolesińska B., Ulański P., “Synthesis and Properties of Targeted Radioisotope Carriers Based on Poly(Acrylic Acid) Nanogels” Pharmaceutics, (2021), 13, 1240.

    13. Maliszewski D., Wróbel A., Kolesinska B., Fraczyk J., Drozdowska D., „1,3,5-Triazine Nitrogen Mustards with Different Peptide Group as Innovative Candidates for AChE and BACE1 Inhibitors” Molecules, (2021), 26, 3942.

    14. Janczewski Ł., Kręgiel D., Kolesińska B., “Synthesis of Isothiocyanates Using DMT/NMM/TsO− as a New Desulfurization Reagent” Molecules, (2021), 26, 2740.

    15. Fraczyk A., Janczewski L., Wasko J., Rozniakowski K., Galecki K., Kaminski Z.J., Kolesinska B., “Non-Aggregating Amylin Fragments as an Inhibitors of the Aggregation Process of Susceptible to Aggregation Fragments 18–22, 23–27, and 33–37 of Hormone” Chem. Biodiversity, (2021) 18.

    16. Rozniakowski K., Galecki K., Wietrzyk J., Filip-Psurska B., Fraczyk J., Kaminski Z.J., Kolesinska B., “N-Methylated Analogs of hIAPP Fragments 18–22, 23–27, 33–37 Inhibit Aggregation of the Amyloidogenic Core of the Hormone” Chem. Biodiversity, (2020), 17.

    17. Wasko J., Wolszczak M., Kaminski Z.J., Steblecka M., Kolesinska B., “Human Serum Albumin Binds Native Insulin and Aggregable Insulin Fragments and Inhibits Their Aggregation”, Biomolecules, (2020),10, 1366.

    18. Rozniakowski K., Fraczyk A., Galecki K., Wietrzyk J., Filip-Psurska B., Fraczyk J., Kaminski Z.J., Kolesinska B., “New Human Islet Amyloid Polypeptide Fragments Susceptible to Aggregation”, Chem. Biodiversity, (2020), 17.

    19. Wasko J., Fraczyk J., Becht A., Kaminski Z.J., Flincec Grgac S., Tarbuk A., Kaminska M., Dudek M., Gliscinska E., Draczynski Z., Kolesinska B., “Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine” Materials, (2020), 13, 3079.

    20. Zielinski D., Fraczyk J., Debowski M., Zielinski M., Kaminski Z.J., Kregiel D., Jacob C., Kolesinska B., “Biological Activity of Hydrophilic Extract of Chlorella vulgaris Grown on Post-Fermentation Leachate from a Biogas Plant Supplied with Stillage and Maize Silage” Molecules, (2020), 25, 1790.

    21. Stodolak-Zych E., Jelen P., Dzierzkowska E., Krok-Borkowicz M., Zych Ł., Bogun M., Rapacz-Kmita A., Kolesinska B., “Modification of chitosan fibers with short peptides as a model of synthetic extracellular matrix” J. Mol. Struct., (2020), 1211, 128061.

    22. Fraczyk J., Wasko J., Walczak M., Kaminski Z.J., Puchowicz D., Kaminska I., Bogun M., Kolasa M., Stodolak-Zych E., Scislowska-Czarnecka A., Kolesinska B., “Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine” Materials, (2020), 13, 337.

    23. Lipinski W.,  Wasko J., Walczak M., Fraczyk J., Kaminski Z.J, Galecki K., Draczynski Z., Krucinska I., Kaminska M., Kolesinska, B., “Fibrous Aggregates of Short Peptides Containing Two Distinct Aromatic Amino Acid Residues”, Chem. Biodiversity, (2019), 16.

    24. Swiontek M., Wasko J., Fraczyk J., Galecki K., Kaminski Z.J., Kolesinska B., “Insulin Hot‐Spot Analogs Formed with N‐Methylated Amino Acid Residues Inhibit Aggregation of Native Hormone” Molecules, (2019), 24, 3706.

    25. Kregiel D., Rygala A., Kolesinska B., Nowacka M., Herc A.S., Kowalewska A., “Antimicrobial and Antibiofilm N-acetyl-L-cysteine Grafted Siloxane Polymers with Potential for Use in Water Systems” Int. J. Mol. Sci.,(2019), 20, 2011.

    26. Chaberska A., Fraczyk J., Wasko J., Rosiak P., Kaminski Z.J., Solecka A., Stodolak-Zych E., Strzempek W., Menaszek E., Dudek M., Niemiec W., Kolesinska B., “Study on the Materials Formed by Self-Assembling Hydrophobic, Aromatic Peptides Dedicated to Be Used for Regenerative Medicine” Chem. Biodiversity, (2019), 16.

    27. Frączyk J., Rosowski A., Kolesińska B., Koperkiewcz A., Sobczyk-Guzenda A., Kaminski Z.J. Dudek M., „Orthogonal Functionalization of Nanodiamond Particles after Laser Modification and Treatment with Aromatic Amine Derivatives” Nanomaterials, (2018), 8, 908.

    28. Kolesińska B., Waśko J., Kamiński Z., Geueke B., Kohler H.-P. E., Seebach D., „Labeling and Protecting N-Terminal Protein Positions by β-Peptidyl Aminopeptidase-Catalyzed Attachment of β-Amino-Acid Residues - Insulin as a First Example” Helv. Chim. Acta, (2018), 101.

    29. Frączyk J., Kamiński Z.J., Katarzyńska J., Kolesińska B., ”4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium Toluene-4-sulfonate (DMT/NMM/TsO-) Universal Coupling Reagent for Synthesis in Solution” Helv. Chim. Acta, (2017), 100.

    30. Świontek M., Rożniakowski K., Frączyk J., Lipiński W., Gałęcki K., Wysocki S., Dupont B.G.R., Kamiński Z.J., Kolesińska B., ”The Quest for the Shortest Fragments of A (13-19) and B (12-17) Responsible for the Aggregation of Human Insulin” Nanomedicine, (2016), 11, 2083-2101.

    31. Świontek M., Kamiński Z.J., Kolesińska B., Seebach D., “Visible-Light Microscopic Discovery of up to 150 μm Long Helical Amyloid Fibrils built of the Dodecapeptide H-(Val-Ala-Leu)4-OH and of Decapeptides Derived from Insulin” Chem. Biodiv., (2016), 13, 1111-1117.

    32. Kolesińska B., Eyer K., Robinson T., Dittrich P.S., Beck A.K., Seebach D., Walde P., “Interaction of β3/β2-Peptides, Consisting of Val-Ala-Leu Segments, with POPC Giant Unilamellar Vesicles (GUVs) and White Blood Cancer Cells (U937) – A New Type of Cell-Penetrating Peptides, and a Surprising Chain Length Dependence of Their Vesicle- and Cell-Lysing Activity” Chem. Biodiv., (2015), 12, 697-732.

    33. Kolesińska B., Rożniakowski K.K., Frączyk J., Relich I., Papini A.M., Kamiński Z.J., „The Effect of Counterion and Tertiary Amine on the Efficiency of N-Triazinylammonium Sulfonates in Solution and Solid-Phase Peptide Synthesis” Eur. J. Org. Chem., (2015), 401-408.

    34. Bąk A., Daszykowski M., Kamiński Z., Kieć- Kononowicz K., Kuder K., Frączyk J., Kolesińska B., Ciosek P., Polański J., „Probing an Artificial Polypeptide Receptor Library Using a Series of Novel Histamine H3 Receptor Ligands” Comb. Chem. & H. T. S., (2014), 17, 141-156.

    35. Sparr C., Purkayastha N., Kolesińska B., Gengenbacher M., Amulic B., Matuschewski K., Seebach D., Kamena F., „Improved Efficacy of Fosmidomycin against Plasmodium and Mycobacterium Species by Combination with the Cell Penetrating Peptide Octaarginine“ Antimicrob. Agents Chemother., (2013), 57, 4689-4698.

    36. . Frączyk J., Kolesińska B., Kamiński Z.J., “N-Lipidated Oligopeptides Immobilized on Cellulose as New Type of Organocatalysts” Comb. Chem. & H. T. S., (2013), 16, 562-571.

    37. . Jastrząbek K., Bednarek P., Kolesińska B., Kamiński Z.J., „Bis(4,6-dimethoxy-1,3,5-triazin-2-yl) Ether as Coupling Reagent for Peptide Synthesis” Chem. Biodiv., (2013) 10, 952-961.

    38. Kolesińska B., Kasperowicz-Frankowska K., Frączyk J., Kamiński Z.J., „Enantioselective Solid Phase Peptide Synthesis Using Traceless Chiral Coupling Reagents and Racemic Amino Acids” Helv. Chim. Acta, (2012), 95, 2084-2098.

    39. Jastrząbek K.G., Subiros-Funosas R., Albericio F., Kolesińska B., Kamiński Z.J., „4-(4,6-Di[2,2,2-trifluoroethoxy]-1,3,5-triazin-2-yl)-4-methylomorpholinium Tetrafluoroborate. Triazine-Based Coupling Reagents Designed for Coupling Sterically Hindered Substrates” J. Org. Chem., (2011), 76, 4506–4513.

    40. Kolesińska B., Kamiński Z.J., „Design, Synthesis and Application of Enantioselective Coupling Reagent with a Traceless Chiral Auxiliary” Org. Lett., (2009), 11, 765-768.

    41. Kamiński Z.J., Kolesińska B., Kolesińska J., Sabatino G., Chelli M., Rovero P., Błaszczyk M., Główka M.L., Papini A.M., „N-Triazinylammonium Tetrafluoroborates. A New Generation of Efficient Coupling Reagents Useful for Peptide Synthesis” J. Am. Chem. Soc., (2005), 127, 16912-16920.

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